<i>In Vitro</i> Antioxidant and Anti-Diabetic Evaluation of a Polyherbal Formulation

Diabetes Mellitus (DM) is a major metabolic disorder causing morbidity and mortality worldwide. The prominent adverse effects of allopathic drugs are the principal reason for an escalating number people seeking alternative complementary-based therapies that may have less or no effects. poly-herbal formulation Vilva Kudineer Choornam (VKC) combination Siddha medicinal plants treatment diabetes. present study aimed to investigate antioxidant antidiabetic efficacy by invitro assays. Aqueous Extract Formulation (AEF) Ethanol (EEF) were used assay. In research work, potential polyherbal was evaluated using DPPH radical scavenging Nitric oxide IC<sub>50</sub> value AEF 333.02 ?g/ml EEF 86.37 in assay whereas, nitric assay, found be 8.61 447.77 ?g/ml, respectively. VKC also screened in-vitro alpha-amylase inhibition it shown significant percentage alpha-amylase. 868.84 ?g/ ml 247.09 findings suggest has activity. Further vitro studies cell lines other enzymes carried out fully establish anti-diabetic formulation.